FIGURE

Fig. S3

ID

ZDB-FIG-130618-36

Publication

Yao et al., 2013 - Anaplastic lymphoma kinase is required for neurogenesis in the developing central nervous system of zebrafish

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Fig. S3

In situ hybridization to detect alk overexpression in alk:HSE:cfp transgenic embryos. A heat shock was performed at 24 hpf. All in situ hybridizations were done using an alk antisense RNA probe, at 1 hour post heat shock (hph) (A), 6 hph (B), 22 hph (C), and shortly after a second HS performed at 48 hpf (D). Inserts show representative transgenic positive embryos. As soon as 1 hour after heat shock, high levels of exogenous alk RNA could be detected in the entire embryo. Reduced staining at 6 hours after HS indicated RNA degradation. Staining almost completely disappeared at 22 hours after HS. A second HS re-activated transcription of overexpressed alk. Arrows points to transgene negative siblings where no exogenous alk was detected. Endogenous alk expression was not detected because of short staining time.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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