FIGURE
            Fig. S4
                
                    
                        Fig. S4
                    
                    
                
                
            
        
        
    
        
            
            
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 ECs of the CCV have no apical polarization (analyzed by Podocalyxin-like 2 expression). (A-E′) Fluorescent confocal images of immunostainings of Podocalyxin 2 (Pdxl2) at the indicated time points; lateral views. The developing vasculature is visualized by transgenic EGFP expression of Tg(kdrl:EGFP)s843. ECs of the CCV do not form Pdxl2-positive membrane compartments, whereas ECs in the fin bud (arrows in D′ and right arrow in E′) and ECs in the aortic arches (left arrow in E′) form apical membrane compartments as indicated by Pdxl2 staining.  | 
    
                
                    
                        Expression Data
                    
                    
                
                
            
        
        
    
        
            
            
            
            
    
    
                
                    
                        Expression Detail
                    
                    
                
                
            
        
        
    
        
            
                
            
        
    
    
    
                
                    
                        Antibody Labeling
                    
                    
                
                
            
        
        
    
        
            
                
            
        
    
    
    
                
                    
                        Phenotype Data
                    
                    
                
                
            
        
        
    
        
            
            
            
            
    
    
                
                    
                        Phenotype Detail
                    
                    
                
                
            
        
        
    
        
            
                
            
        
    
    
    
                
                    
                        Acknowledgments
                    
                    
                
                
            
        
        
    
        
            
            
                
                    
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