FIGURE

Fig. 8

ID

ZDB-FIG-131105-24

Publication

Rosenfeld et al., 2013 - Small heat shock proteins Hspb7 and Hspb12 regulate early steps of cardiac morphogenesis

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Fig. 8

Hspb7 is required in Kupffer′s vesicle for normal morphogenesis. (A, B) Confocal stack and DIC images, respectively, of the KV in wildtype and morphant embryos derived from the sox17:gfp reporter strain, as indicated at the 8–10 somite stage. In this case, embryos were injected with MOs at the 1K stage, and selected for YSL-specific deposition using a reporter dye. Embryos in (A) were stained with anti-acetylated tubulin antibody (red). The bars represent 10 µm. (C–E) Quantification of KV phenotypes noting average cilia number (C), cilia length (D) or KV width (E). In each sample n was at least five embryos. The asterisk indicates p<0.05 according to Student′s t-test in comparison to the standard control group.

Expression Data

Gene:	EGFP
Fish:	s870Tg s870Tg + MO1-hspb7 s870Tg + MO1-hspb12
Knockdown Reagents:	MO1-hspb7 MO1-hspb12
Anatomical Term:	Kupffer's vesicle
Stage Range:	5-9 somites to 10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	s870Tg + MO1-hspb7 s870Tg + MO1-hspb12
Knockdown Reagents:	MO1-hspb7 MO1-hspb12
Observed In:	Kupffer's vesicle Kupffer's vesicle ciliated cell
Stage Range:	5-9 somites to 10-13 somites

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 381(2), Rosenfeld, G.E., Mercer, E.J., Mason, C.E., and Evans, T., Small heat shock proteins Hspb7 and Hspb12 regulate early steps of cardiac morphogenesis, 389-400, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.