Fig. 4

Slc7a7⁺ Cells Give Rise to Microglia in the Brain

(A–C) Lateral view shows a representative slc7a7::Kaede;fms::nfsB-mCherry embryo at 2 days post fertilization; slc7a7⁺ cells (A, green, slc7a7::Kaede), macrophages (B, red, fms::nfsB-mCherry), and overlay (C) are shown. Few cells are both fms⁺ and slc7a7⁺ (yellow arrowheads). White arrowheads indicate fms⁺ cells only.

(D–F) Frontal view shows a representative slc7a7::Kaede;pU.1::TagRFP embryo at 2 days post fertilization. Slc7a7⁺ cells (D, green, slc7a7::Kaede), leukocytes (E, red, pU.1::TagRFP), and overlay (F) are shown. Few cells are both pU.1⁺ and slc7a7⁺ (yellow arrowheads). White arrowheads indicate pU.1⁺ cells only.

(G–I) Dorsal view shows a slc7a7::Kaede;pU.1::TagRFP embryo at 4 days post fertilization. Microglia are both slc7a7⁺(G) and pU.1⁺ (H), with overlay (I) and a representative microglia (inset) shown.

(J–O) Dorsal view shows a 4-day-post-fertilization slc7a7::Kaede embryo not photoconverted (J–L) and photoconverted at 24 hpf (M–O) (Kaede green, J and M; Kaede red, K and N; overlay, L and O).

(P–R) Representative example of a photoconverted microglia is shown.

Scale bars for all images, 25 µm. See also Figure S4.