FIGURE

Fig. S11

ID

ZDB-FIG-171227-20

Publication

Shao et al., 2017 - Vegetally localised Vrtn functions as a novel repressor to modulate bmp2b transcription during dorsoventral patterning in zebrafish.

Other Figures

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Fig. S11

Somite number is reduced in vrtn morphants or mutants. (A-D) Specificity of the vrtnMO. Injection of vrtnMO efficiently blocked the translation of the artificial mRNA where the vrtnMO-targeting 5' leader sequence is fused with vrtn-GFP. It has no effect on the artificial mRNA where only vrtn coding region is fused with GFP sequence. (E,F) Phenotypes of uninjected and vrtnMO-injected embryos at 30 hpf. (G) Analysis of the number of somite boundary at 36 hpf after ISH using dystrophin as a marker. (H,I) Statistical analysis of somite number in vrtn morphants and mutants. Horizontal lines represent the mean values ± s.d. from four batches of embryos. Scale bars: (A-D) 400 μm; (E-G) 250 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	vrtn^sdu1/sdu1 WT + MO1-vrtn
Knockdown Reagent:	MO1-vrtn
Observed In:	somite
Stage:	Prim-25

Phenotype Detail

Acknowledgments

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