Fig. 4

Body size and lipid amounts are affected by doc2a and fam57ba. A representative experiment is shown from the 5 conducted. (A,B) 12 dpf larvae stained with Oil Red O (ORO) are shown for wild-type and doc2a^+/? fam57ba^+/?. (C) A significant increase in body length as measured from the tip of the nose to the end of the tail is found for doc2a^+/? fam57ba^+/? larvae (n?=?19) compared to wild-type (n?=?19). (D) ORO was extracted back out of the stained larvae and quantified, showing a significant increase in the concentration of lipids in the double heterozygotes (yellow bar) compared to wild-type (blue bar). (E) After accounting for length, there is no longer a significant increase in ORO concentration in doc2a^+/? fam57ba^+/? larvae. (F,G) Wild-type and fam57ba^?/? larvae stained with ORO. (H) A significant increase in body length was found comparing fam57ba^?/? larvae (n?=?30) to wild-type (n?=?30). (I) ORO quantification showed a significant increase in the concentration of lipids for fam57ba^?/? homozygotes (purple bar) compared to wild-type (blue bar). (J) When accounting for length, ORO concentration is still significantly elevated in fam57ba^?/? fish. (K?Y) No significant changes in size or ORO concentration were observed for fam57ba^+/? (n?=?29), doc2a^+/? (n?=?26), and doc2a^?/? (n?=?36) larvae compared to their respective wild-type controls. **P?<?0.01, ***P?<?0.001, ****P?<?0.0001. Scale bar?=?500 µm.