FIGURE

Fig. 2

ID

ZDB-FIG-180221-21

Publication

Piatek et al., 2017 - Ectopically expressed Slc34a2a sense-antisense transcripts cause a cerebellar phenotype in zebrafish embryos depending on RNA complementarity and Dicer

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Fig. 2

Injection of Slc34a2a RNA into fertilized zebrafish eggs.

A) Visual classification of malformations depending on the severity of the defect: Level 1, wild type; level 2, one organ affected (size, shape or function, e.g. heart rate); level 3, 2–3 organs affected, level 4, multiple malformations; level 5, developmental arrest. B) Phenotypic characterization of zebrafish embryos injected with various RNAs, Slc34a2a (554 embryos in total), antisense (94 embryos) and Slc34a2b (538 embryos). C) (i) Anatomy of a 48 hpf zebrafish embryo: Y, yolk sac; E, eye; O, otic vesicle (ear); R1/R7, rhombomeres; M, mesencephalon; C, cerebellum, in red. (ii) non injected wild type embryo; (iii) Slc34a2a injected embryo; (iv) wild type embryo, Eng2 stained; (v) Slc34a2a injected embryo, Eng2 stained; (vi, vii) Slc34a2a(as) and Slc34a2b Eng2 stained.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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