FIGURE

Figure 8

ID

ZDB-FIG-201221-8

Publication

Qian et al., 2020 - The slc4a2b gene is required for hair cell development in zebrafish

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Figure 8

slc4a2b mRNA injection can rescue the phenotype induced by slc4a2b gene deficiency. The Tg(Brn3c:mGFP);slc4a2b^mut zebrafish that were injected with Cas9 mRNA and slc4a2b sgRNA had fewer HC clusters (A, B) and fewer HCs in each posterior lateral line neuromast (A, C) compared to controls. However, slc4a2b mRNA injection could rescue the phenotype caused by slc4a2b mutation. *** P < 0.001; ** P < 0.01; ns, no significance.

Expression Data

Antibody:	Ab16-GFP
Fish:	slc4a2b^{zf4069/zf4069}; s356tTg (AB) slc4a2b^{zf4069/zf4069}; s356tTg + CRISPR1-slc4a2b (AB)
Knockdown Reagent:	CRISPR1-slc4a2b
Anatomical Term:	posterior lateral line neuromast hair cell
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	slc4a2b^{zf4069/zf4069}; s356tTg (AB) slc4a2b^{zf4069/zf4069}; s356tTg + CRISPR1-slc4a2b (AB)
Knockdown Reagent:	CRISPR1-slc4a2b
Observed In:	neuromast neuromast hair cell posterior lateral line neuromast hair cell
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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