FIGURE

Figure 4

ID

ZDB-FIG-210530-11

Publication

Xie et al., 2021 - Glucocorticoid-Induced Exacerbation of Mycobacterial Infection Is Associated With a Reduced Phagocytic Capacity of Macrophages

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Figure 4

Effect of beclomethasone on gene expression levels in FACS-sorted macrophages. At 28 hpf, Tg(mpeg1:mCherry-F) embryos were treated with vehicle or beclomethasone for two hours, after which macrophages were isolated by FACS sorting. Gene expression levels were determined in the sorted cells by qPCR for tnfa(A), sparcl1(B), uchl1(C), ube2v1(D), marcksa(E), marcksb(F), bsg(G) and tubb5(H). Statistical analysis by two-tailed t-test showed that the levels of tnfa, sparcl1, uchl1, marcksa and marcksb expression were significantly inhibited by beclomethasone treatment. Data shown are the means ± s.e.m. of three or four independent experiments, and markers show averages of individual experiments. Statistical significance is indicated by: ns, non-significant; *P<0.05; ***P<0.001.

Expression Data

Genes:	marcksa marcksb sparcl1 tnfa uchl1
Fish:	ump2Tg/ump2Tg
Condition:	chemical treatment by environment: beclomethasone
Anatomical Term:	neutrophil
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ump2Tg/ump2Tg
Condition:	chemical treatment by environment: beclomethasone
Observed In:	neutrophil
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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