FIGURE

Figure 3

ID

ZDB-FIG-210606-78

Publication

Hung et al., 2021 - Proapoptotic Bad Involved in Brain Development, When Severely Defected, Induces Dramatic Malformation in Zebrafish

Other Figures

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Figure 3

Bad knockdown can enhance ROS production and induces oxidative stress at early embryonic stage. (A) Loss-of-Bad-mediated cell death can enhance ROS production. ROS production was assayed using H2DCFDA in embryos with Bad knockdown at 8 hpf. The green fluorescent signal, reflecting ROS, is indicated by arrows. Loss of PCD can enhance environmental stress in blue cells; (Ad3) due to ROS production. Bars indicate 100 μm. (B) Analysis of oxidative stress response-related genes by qRT-PCR at 8 hpf with Bad knockdown. All data were analyzed using either paired or unpaired Student’s t-tests, as appropriate.

Expression Data

Genes:	cat nfe2l2a nfe2l2b sod1 sod2
Fish:	AB AB + MO4-badb
Knockdown Reagent:	MO4-badb
Anatomical Term:	whole organism
Stage:	75%-epiboly

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO4-badb
Knockdown Reagent:	MO4-badb
Observed In:	reactive oxygen species biosynthetic process whole organism
Stage:	75%-epiboly

Phenotype Detail

Acknowledgments

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