FIGURE

Figure 3

ID

ZDB-FIG-210923-26

Publication

Li et al., 2021 - Developmental Angiogenesis Requires the Mitochondrial Phenylalanyl-tRNA Synthetase

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Figure 3

The deficiency of FARS2 impairs cell motility, proliferation, invasion, and tube formation in human umbilical vein endothelial cells (HUVECs). (A) Representative images of scratch-wound assays of HUVECs 0 and 6 h at 48 h after transfection with a control (siCtrl) or FARS2-specific (si-FARS2) siRNA. Scale bar = 200 μm. (B) Quantification of the healed wound area from (A). Data are prepresented as the mean and SEM (n = 10). ^***P < 0.001 via ANOVA. (C) A CCK8-based cell proliferation assay of HUVECs at the indicated time-points after transfection with siCtrl or si-FARS2. The measurements were made in triplicate (mean and SEM), and the results are indicative of three independent experiments. ^****P < 0.0001. (D) Representative images of transwell-based migration assays of HUVECs 48 h after transfection with siCtrl or si-FARS2. Scale bar = 200 μm. (E) Quantification of the number of migrated cells from (D). ^***P < 0.001. (F) Representative images of tube network assays of HUVECs 48 h after transfection with siCtrl or si-FARS2. Scale bar = 200 μm. (G,H) Quantification of the branching points (G) and tube lengths (H) from (F). The measurements were made in triplicate (mean and SEM), and the results are indicative of three independent experiments. ^****P < 0.0001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cardiovasc Med