Fig. 8

Summary of BB behavior and Par3 localization during early and late stages of development in the FP of wt and vangl2 zebrafish mutants. In wt embryos during 4-8s stages, the BBs, which anchor a growing cilium, shuttle between anterior and posterior cell apical junctions, and contact membranes only at the level of Par3 patches. Par3 becomes progressively enriched at posterior junctions. By 18-22s stages, the vast majority of BBs are stably docked to a posterior patch of Par3. BBs from vangl2−/− embryos during the 4-8s stages are motile and make poorly oriented membrane contacts. They still contact Par3 patches, which are fragmented and equally distributed between lateral and antero-posterior membranes. By the 18-22s stages, vangl2−/− BBs occupy a central position within the apical surface.