FIGURE

Figure 2

ID

ZDB-FIG-220316-3

Publication

Cotti et al., 2022 - Compression Fractures and Partial Phenotype Rescue With a Low Phosphorus Diet in the Chihuahua Zebrafish Osteogenesis Imperfecta Model

Other Figures

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Figure 2

Vertebral body shape variation in Chi/+ and Chi/+_LP mutants. (A) Alizarin red S stained vertebral body of a WT animal with 2D landmark positions used for quantifying the shape variation by means of geometric morphometrics, represented in (B). (B) The scatterplot of WT, Chi/+ and Chi/+_LP 2D landmarks shows high variation in the superimposition of X,Y Procrustes coordinates of Chi/+ compared to WT animals. Chi/+_LP animals display reduced landmark variation compared to Chi/+ and a distribution more similar to WT indicating a partial rescue of shape variation at three months of age. The first 10 caudal vertebral centra in WT (n=15), Chi/+ (n=13) and Chi/+LP (n=12) were analysed. The 95% confidence ellipses are shown. (C) Principal component analysis of vertebral centra shapes. Each symbol in the plot represents a vertebral body. PC indicates Principal Component and the values in the axis labels indicate the percentage of variation accounted for by each axis. Chi/+ animals show high variance compared to WT animals (Chi/+ versus WT, PC1 0.3321, PC2 0.1795; Chi-square test: p < 0.001). Variance is rescued in Chi/+_LP animals (Chi/+_LP versus WT, PC1 0.2484, PC2 0.2020; Chi-square test: non-significant). The 95% confidence ellipses are shown.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	col1a1a^dc124/+ (AB)
Observed In:	vertebra
Stage:	Days 21-29

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Endocrinol (Lausanne)