FIGURE

Fig. 6

ID

ZDB-FIG-221223-6

Publication

Lei et al., 2021 - Noncanonical protease-activated receptor 1 regulates lymphatic differentiation in zebrafish

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Fig. 6

Overexpression of cleaved par1 recovers the lymphatic phenotypes observed in mmp13b zebrafish mutants

(A) Confocal images showing TD formation of siblings and mmp13b homozygous mutants in the Tg(lyve1b:mcherry) line and Tg(lyve1b:cleaved-par1-P2A-mcherry) line at 5 dpf. Blue arrowheads indicate TD formation in each somite; yellow asterisks represent the absence of TD formation in each somite. Scale bars: 100 μm.

(B) Percentage of somites lacking TD formation in siblings with the Tg(lyve1b:mcherry) line (n = 30 embryos), mmp13b homozygous mutants with the Tg(lyve1b:mcherry) line (n = 20 embryos), siblings with the Tg(lyve1b:cleaved-par1-P2A-mcherry) line (n = 30 embryos), and mmp13b homozygous mutants with the Tg(lyve1b:cleaved-par1-P2A-mcherry) line (n = 20 embryos); 6 somites/embryos were used for quantification. In (B), values represent means ± SEMs. ∗p ≤ 0.01, ∗∗p ≤ 0.001 in the Student's t test.

Expression Data

Gene:	mCherry
Fish:	zf3743Tg/zf3743Tg zf3744Tg/zf3744Tg mmp13b^{zf3746/zf3746}; zf3743Tg/zf3743Tg mmp13b^{zf3746/zf3746}; zf3744Tg/zf3744Tg
Anatomical Term:	thoracic duct
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mmp13b^{zf3746/zf3746}; zf3743Tg/zf3743Tg mmp13b^{zf3746/zf3746}; zf3744Tg/zf3744Tg
Observed In:	thoracic duct
Stage:	Day 5

Phenotype Detail

Acknowledgments

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