Figure 3
- ID
- ZDB-FIG-230211-132
- Publication
- Morgan et al., 2023 - ahctf1 and kras mutations combine to amplify oncogenic stress and restrict liver overgrowth in a zebrafish model of hepatocellular carcinoma
- Other Figures
- All Figure Page
- Back to All Figure Page
(a) Representative Airyscan imaging of liver cryosections stained with α-tubulin antibody (magenta) marking spindle microtubules and DAPI (cyan) marking DNA in mitotic cells of TO(krasG12V)T/+ larvae on a wildtype ahctf1+/+ background. (b) Mitotic cells in liver cryosections of TO(krasG12V)T/+ larvae that are heterozygous for ahctf1+/− exhibit multiple defects, including multipolar spindles, misaligned chromosomes, and anaphase bridges (arrows). Scale bar 2 µm. (c) Distribution of cells observed at different mitotic stages (n = 92 livers, 326 mitotic cells). (d) Quantification of the percentage of mitotic hepatocytes exhibiting an aberrant phenotype (n = 14–57). Significance was assessed using a Chi-square test.
|
| Antibody: | |
|---|---|
| Fish: | |
| Condition: | |
| Anatomical Term: | |
| Stage: | Days 7-13 |
| Fish: | |
|---|---|
| Condition: | |
| Observed In: | |
| Stage: | Days 7-13 |