FIGURE

Figure 3

ID

ZDB-FIG-230228-353

Publication

Trejo-Reveles et al., 2023 - Identification of Novel Coloboma Candidate Genes through Conserved Gene Expression Analyses across Four Vertebrate Species

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Figure 3

Spatial gene expression analysis for 10 conserved fissure-enriched genes in the developing chick eye. Fluorescent in situ hybridisation for (a) known coloboma gene targets and (b) OFC candidate genes identified in this study was counterstained with DAPI nuclear stain on chick eyes at HH28, immediately prior to fusion. Note that for EMX2, the OFM region highlights the POM region; expression was not observed in the NR or RPE. In both (a,b), Top: grayscale RNAscope probe signal without DAPI highlighting the mRNA signal in the immediate optic fissure margin region. Middle: mRNA probe and DAPI merged images of the broad ventral eye region. Bottom: Dorsal eye region with probe and DAPI merged. OFM, optic fissure margin; NR, neural retina; POM, periocular mesenchyme; RPE, retinal pigmented epithelium. Scale = 50 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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