FIGURE

Fig. 1

ID

ZDB-FIG-230405-8

Publication

Tan et al., 2022 - Pax2a, Sp5a and Sp5l act downstream of Fgf and Wnt to coordinate sensory-neural patterning in the inner ear

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Fig. 1

Early responses to modulation of Wnt and Fgf. (A–J) Dorsal views (anterior to the top) showing expression at 14 hpf of the artificial Wnt reporter top:gfp (A–E) and the Fgf-target gene etv5b (F–J) following treatment at 12 hpf with DMSO (control), hs:dkk1 (39 °C for 1 h) + 10 μM IWR-1 added immediately after heat shock, BIO (5 μM), SU5402 (70 μM), or hs:fgf8a (39 °C for 1 h). Epiphysis (epi) and midbrain-hindbrain border (MHB) are indicated. Brackets mark a zone of weaker top:gfp expression in the hindbrain. The same conditions were used in subsequent figures, except for changes in treatment duration and time of fixation as noted.

Expression Data

Genes:	etv5b GFP
Fish:	w25Tg/w25Tg AB w32Tg/w32Tg x17Tg/x17Tg w25Tg/w25Tg; w32Tg/w32Tg w25Tg/w25Tg; x17Tg/x17Tg
Conditions:	chemical treatment by environment: 6-bromoindirubin-3'-oxime chemical treatment by environment: IWR-1-endo chemical treatment by environment: SU5402
Anatomical Term:	central nervous system
Stage Range:	5-9 somites to 10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	w25Tg/w25Tg AB w32Tg/w32Tg x17Tg/x17Tg w25Tg/w25Tg; w32Tg/w32Tg
Conditions:	chemical treatment by environment: 6-bromoindirubin-3'-oxime chemical treatment by environment: IWR-1-endo chemical treatment by environment: SU5402
Observed In:	central nervous system
Stage Range:	5-9 somites to 10-13 somites

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 492, Tan, A.L., Mohanty, S., Guo, J., Lekven, A.C., Riley, B.B., Pax2a, Sp5a and Sp5l act downstream of Fgf and Wnt to coordinate sensory-neural patterning in the inner ear, 139-153, Copyright (2022) with permission from Elsevier. Full text @ Dev. Biol.