Fig. 2

Zebrafish Abcb4 is an efflux transporter of rhodamine123, a substrate for human Pgp. a Fluorescence micrographs indicate the accumulation rhodamine123 in the WT and abcb4 knockout embryos. The intensity of rhodamine123 dye in WT and abcb4 knockout embryos was quantified and illustrated as a bar graph b. c Protocol of drug treatment for vinblastine and doxorubicin toxicity experiments. e Representative images of embryos with defects development after vinblastine treatment. d The percentage of embryos showing developmental abnormalities for WT and abcb4 mutant in the presence and absence of 2 μM vinblastine was compared by two-way ANOVA. eabcb4 knockout embryos treated with 100 µM doxorubicin demonstrate a reduction of body length in a two-tailed t-test. Data are expressed as mean ± SD from at least three independent experiments. n numbers indicate the total number of embryos across the independent experiments