FIGURE

Fig. 7

ID

ZDB-FIG-231215-15

Publication

Zhang et al., 2022 - Blf and drl cluster synergistically regulate cell fate commitment during zebrafish primitive hematopoiesis

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Fig. 7

Zfp932 is a potential mammalian functional ortholog of the zebrafish blf and drl cluster genes. (A) qPCR of the erythroid marker gene hbae1 and the neutrophil marker gene mpx in blf−/−; drl 4KO embryos after injection of human or mouse mRNA. Data are mean±s.e.m. of at least three replicates. **P<0.01. (B) Left: Expression of hbae1 in 23 hpf embryos; n=27, 32 and 29 for each experimental set. Middle: Whole-mount o-dianisidine staining of 36 hpf embryos; n=31, 39 and 33 for each experimental set. Ventral views. Right three panels: RNA in situ hybridization of mpx, runx1 and c-myb expression. For mpx WISH, n=38, 33 and 25. For runx1 WISH, n=38, 29 and 30. For c-myb WISH, n=31, 25 and 37. Listed sample sizes relate to each group shown from top to bottom. Scale bars: 100 μm.

Expression Data

Genes:	hbae1.1 mpx myb runx1
Fish:	blf^{nju210Tg/nju210Tg}; Df(Chr5:drl,drll.1,drll.2,drll.3)nju212/nju212
Anatomical Terms:	dorsal aorta erythroid progenitor cell neutrophil progenitor cell
Stage Range:	26+ somites to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	blf^{nju210Tg/nju210Tg}; Df(Chr5:drl,drll.1,drll.2,drll.3)nju212/nju212
Observed In:	dorsal aorta erythroid progenitor cell neutrophil progenitor cell
Stage Range:	26+ somites to Prim-25

Phenotype Detail

Acknowledgments

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