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The generation of 2 AC types requires distinct sets of TFs. (A-E) Representative images showing the distributions of 2 GABAergic/cholinergic ACs labeling by TgBAC(sox2: mNeonGreen,bhlhe23:mRuby3) after injection of control sgRNA(A), bhlhe22 sgRNA (B, bhlhe22 disruption), bhlhe23 sgRNA (C, bhlhe23 disruption), bhlhe22/bhlhe23 sgRNA (D, bhlhe22 and bhlhe23 codisruption), sox2 sgRNA (E, sox2 disruption) at 5 dpf. (F) Quantifications of sox2+ type in (A-E). (G) Quantifications of bhlhe22 + type in (A-E). The data underlying this figure can be found in S3 Data. Data are presented as mean ± SD, Mann–Whitney test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Scale bars, 10 μm. AC, amacrine cell; dpf, days post-fertilization; GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; sgRNA, small guide RNA; TF, transcription factor.
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