FIGURE

Fig 8

ID

ZDB-FIG-240614-70

Publication

Belt et al., 2024 - Myeloid Targeted Human MLL-ENL and MLL-AF9 Induces cdk9 and bcl2 Expression in Zebrafish Embryos

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Fig 8

Venetoclax and Flavopiridol co-treatment significantly reduced MLL expression on the yolk of lyz:MLL-ENL and lyz:MLL-AF9 injected embryos.

Lyz:MLL-ENL injected embryos were incubated with 200nM DMSO (A, N = 82), 200nM Venetoclax (B, N = 76), 200nM Flavopiridol (C, N = 84) or 200nM Venetoclax and Flavopiridol (D, N = 98) at 24 hpf and the number of MLL positive cells was assessed at 72 hpf (E). Lyz:MLL-AF9 injected embryos were incubated with 200nM DMSO (F, N = 96), 200nM Venetoclax (G, N = 105), 200nM Flavopiridol (H, N = 102) or 200nM Venetoclax and Flavopiridol (I, N = 95) at 24 hpf and the number of MLL positive cells was assessed at 72 hpf (E,J). Lyz:MLL-ENL (K,L) and lyz:MLL-AF9 (M,N) injected embryos were assessed for expression of tp53 and mcl1a by qPCR (N = 2). Statistical analysis was conducted using one-way ANOVA followed by Tukey HSD. * p<0.05. Anterior to the left.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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