FIGURE

Figure 3

ID

ZDB-FIG-241115-118

Publication

Zhao et al., 2024 - The Glutamine Synthetases Are Required for Sensory Hair Cell Formation and Auditory Function in Zebrafish

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Figure 3

The knockdown of glulb expression resulted in a delayed response of zebrafish to both vibrational and auditory stimuli. (A) Schematic representation of the glulb-MO binding site and potential cleavage methodologies. (B) Successful glulb gene knockdown validated by PCR analysis. (C) Diagram illustrating the experimental setup for vibration stimulation: 7-day-old juvenile zebrafish were positioned within the mold, exposed to vibration stimulation, and subsequently subjected to data recording and analysis. (D,E) Statistical analysis revealed a significant decrease in the distance traveled and swimming velocity of zebrafish in the glulb-MO group compared to the Ctrl group. t-test: *, p < 0.05; ****, p < 0.0001. (F) Five-day-old zebrafish juveniles were placed in the orifice plate, exposed to acoustic stimulus, and subjected to subsequent data recording and analysis. (G–I) The glulb-MO group exhibited a significant reduction in the movement distance, average speed, and sustained movement time of zebrafish compared to the control group, as revealed by statistical analysis. t-test: ****, p < 0.0001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO2-glulb
Conditions:	acoustic radiation vibration
Knockdown Reagent:	MO2-glulb
Observed In:	swimming behavior
Stage Range:	Day 5 to Days 7-13

Phenotype Detail

Acknowledgments

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