Fig. 2

H2O2 induces nociceptive-like behaviour in zebrafish larvae via TRPA1. (a) Typical traces, concentration–response curve and cumulative data of calcium responses in zTRPA1b-HEK293T cells exposed to H2O2 or vehicle (Veh) and in the presence of A-967079 (A96, 30 μM) (cell numbers: H2O2: 3 μM n = 120, 10 μM n = 93, 30 μM n = 78, 100 μM n = 81, 300 μM n = 90, 1 mM n = 83, 3 mM n = 90, 10 mM n = 99, A96 = 110, Veh = 77). (b,c) Typical traces and cumulative data of the locomotor activity of 5 days post fertilization (dpf) zebrafish larvae exposed to H2O2 (100–500 μM, 1 mM) or Veh and in the presence of A96 (10 μM) (n = 8–15 larvae per group). (d) Typical images and dose-dependent writhing behaviour in 24–27 post fertilization (hpf) zebrafish larvae exposed to H2O2 (30–100–300 mM) or Veh and in the presence of A96 (30 μM) (n = 60 larvae per group). (e) Typical images and dose-dependent light touch response in 24–27 hpf zebrafish larvae exposed to H2O2 (30–100–300 mM) or Veh and in the presence of A96 (30 μM) (n = 60 larvae per group). Mean ± SEM. *P < 0.05; #P < 0.05 one-way ANOVA and Bonferroni post hoc test.