Fig. 6

Zebrafish Abcb6 MO15 morphants show altered behavior compared to uninjected zebrafish.

Zebrafish movement paths overlaid with last video frame shows uninjected zebrafish swim smoothly in the upper 2/3 of the tank (a), while the zebrafish Abcb6 MO15 morphants swim erratically and spend a significant amount of time in the lower third of the tank (b). Abcb6 MO15 morphants (purple) swam further with each video frame (c), p = < 0.0001, and swam farther from their starting position (d), p = 0.013, than the uninjected controls (indigo). Abcb6 MO15 morphants also swam longer routes than their uninjected counterparts (e), p = 0.0176, and spent a significantly higher percentage of time in the bottom of their tank (39.9%) compared to the uninjected control (1.2%) (f), p = < 0.0001). Although there was no significant difference in total horizontal distance traveled (g) p = 0.0785, Abcb6 MO15 morphants traveled a greater vertical distance (160.7 pixels) than their uninjected controls (79.7 pixels, (h), p = 0.0041. * = p < 0.05, ** = p < 0.01, **** = p < 0.0001, ns = not significant by unpaired two-tailed Welch’s T-test compared to uninjected control. Data from 21 dpf AB zebrafish, N = 18 fish for uninjected, N = 25 fish for Abcb6 MO15. Median is shown as a dashed line and upper and lower quartiles are shown as dotted lines. Source data are provided in the Source Data file.