FIGURE

Figure 2

ID

ZDB-FIG-241129-49

Publication

Lazcano et al., 2024 - Ontogeny of Thyroid Hormone Signaling in the Retina of Zebrafish: Effects of Thyroidal Status on Retinal Morphology, Cell Survival, and Color Preference

Other Figures

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Figure 2

Comparison of dio3b and thrb crispants at retinal level. (A) Electropherograms from a fragment of the PCR amplicon sequence for dio3b and thrb of the vehicle-microinjected controls and the corresponding crispants. At the top, the sgRNA sequence is denoted, and the PAM sequence is highlighted in red. In the crispants, a decrease in the resolution of the electropherogram is evident around the canonical cutting site indicated with an arrow and a dotted line. (B) Representative histological cross-sections of retinas of vehicle-injected controls and thrb and dio3 crispants (5–7 retinas per condition) stained with H&E. (C) Amplification of the GCL (insets in (B)). (D) Quantification of the number of cells/1000 µm². ONL = outer nuclear layer, INL = inner nuclear layer, GCL = ganglion cell layer. Statistical analyses were performed using one-way ANOVA and Tukey post hoc tests. * = p < 0.05. Scale bar = 20 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + CRISPR1-dio3b WT + CRISPR1-thrb
Knockdown Reagents:	CRISPR1-dio3b CRISPR1-thrb
Observed In:	retinal ganglion cell layer retinal ganglion cell
Stage:	Day 5

Phenotype Detail

Acknowledgments

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