IGF2BP2 and LGALS8 regulate vascular development in the zebrafish model. (A,B) Schematic of CRISPR/Cas9-mediated generation of igf2bp2a/b crispant zebrafish and identification via RNA-sequencing. (C) Representative images of cerebrovascular structures and (D) statistical analysis of mid- and hind-brain vascular areas in control and igf2bp2a/b crispant zebrafish. (E) Representative images of cerebrovascular structures and (F) statistical analysis of mid- and hind-brain vascular areas in igf2bp2a/b crispant and rescue zebrafish. (G,H) Schematic of CRISPR/Cas9-mediated generation of lgals8a/b crispant zebrafish and identification via RNA-sequencing. (I) Representative images of cerebrovascular structures and (J) statistical analysis of mid- and hind-brain vascular areas in control and lgals8a/b crispant zebrafish. (K) Representative images of cerebrovascular structures and (L) statistical analysis of mid- and hind-brain vascular areas in lgals8a/b crispant and rescue zebrafish. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.
|
