FIGURE

Figure 1

ID

ZDB-FIG-250127-152

Publication

Prill et al., 2025 - Heart Morphogenesis Requires Smyd1b for Proper Incorporation of the Second Heart Field in Zebrafish

Other Figures

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Figure 1

Still heart mutant hearts do not loop and display an expansion of cardiac jelly. At 48 hpf, wild-type hearts have looped, with the ventricle and atrium chambers in different planes of focus (A). Unlike wild-type, our still heart mutant hearts do not loop, which allows for easy visualization of both chambers in a single plane of focus at 48 hpf (B). Wild-type hearts (C) display a thin layer of cardiac jelly (CJ) between the endocardial (END) and myocardial (MYO) layers when compared to still heart mutant hearts (D). Panels C and D are magnifications of the atrial walls from panels A and B, respectively. Quantitative PCR analysis of hyaluronan synthase 2 (has2) at 48 hpf showed that the relative fold change in expression of has2 was significantly higher in still heart mutants when compared to wild-type embryos (E). *** = p ≤ 0.001.

Expression Data

Gene:	has2
Fish:	WT smyd1b^{tm123a/tm123a}
Anatomical Term:	whole organism
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	smyd1b^{tm123a/tm123a}
Observed In:	cardiac jelly heart heart looping whole organism
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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