Fig. 2
- ID
- ZDB-FIG-250416-88
- Publication
- Jia et al., 2025 - Activation of prep expression by Tet2 promotes the proliferation of bipotential progenitor cells during liver regeneration
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Prep is required for liver regeneration. (A) Confocal microscope projection images showing the size of the regenerating livers of WT siblings and Prep homozygous mutants (Prep−/−) at BT, R0h, R8h, R24h and R48h. Right panel: area of fluorescence statistics of Dendra2. (B) Brightfield and epifluorescence merged images showing the body phenotypes of WT siblings and Prep−/− at R48h. (C) Top panel: experimental scheme illustrating the pattern of the Prep inhibitor PRA added at 4 dpf and 5 dpf. Bottom panel: confocal microscope projection images showing the size of regenerating livers in the DMSO and PRA treatment groups at R24h and R48h. (D) Top panel: experimental scheme illustrating the pattern of the Prep inhibitor PRA added at R0h and R48h. Bottom panel: confocal microscope projection images showing the size of regenerating livers from the DMSO and PRA treatment groups at R24h and R48h. (E) Area of fluorescence statistics of Dendra2 for C and D. (F) Confocal microscope projection images showing the size of the regenerating livers of WT siblings and Tg (hsp701: Prep) at BT, R0h, R8h, R24h, and R48h. Right panel: area of fluorescence statistics of Dendra2. The observed green fluorescence is derived from the Denra2 fluorescent protein, specifically labeling the liver of zebrafish. Ns, not significant; *P<0.05, **P<0.01, ***P<0.001 (two-tailed post hoc test). Data are mean±s.e.m. Scale bars: 100 µm (A,C,D,F); 500 µm (B). |