Fig. 6

Metrns and ItgαV/Itgβ1b act together for the proper clustering and migration of DFCs. (A) Quantification in percentage of embryos with DFC clustering defects at 9 hpf in non-injected wild type (WT), WT +2.5 ng control morpholino (WT +ctrl MO), WT +insufficient doses of itgαV/itgβ1b MOs (WT +0.41 ng itgαV; WT +0.5 ng itgβ1b), WT +sufficient itgαV/itgβ1b MO doses (WT +1.25 ng itgαV; WT +0.7 ng itgβ1b), triplMut+/- non-injected, triplMut+/- + 2.5 ng ctrl MO and triplMut+/- with insufficient doses of itgαV/itgβ1b MOs (triplMut+/- + 0.41 ng itgαV; triplMut+/- + 0.5 ng itgβ1b). Fisher exact tests indicate *p-value <0.01, **p-value <0.001 and ***p-value <0.0001 and ns = not significant. Error bars indicate standard deviation. (B) Schematic representation modeling the novel role of Metrns for DFCs assembly and migration, for the proper KV formation and subsequent Nodal factors expression distribution at the base of the correct L-R axis establishment during early development. Created with BioRender.com.