Rab11a deficient induces phenotypic anomalies in zebrafish larvae (A) Representative images of zebrafish larvae expressing HuC:eGFP. Brain fluorescence pattern at 5 dpf is displayed (dorsal view). Left, control injected with cas9; right, rab11a crispant. The white dashed line shows the outline of the CNS, which is divided into three parts: forebrain, midbrain, and hindbrain. (B–D) Measurements of brain fluorescence area in control vs rab11a crispant. (B) comparison of brain area data distribution between Cas9 control group and rab11a crispant group; (C) comparison of forebrain area data and frequency distribution between Cas9 control group and rab11a crispant group; (D) comparison of midbrain data between Cas9 control group and rab11a crispant group. (E) comparison of hindbrain data between Cas9 control group and rab11a crispant group. (F,G) Representative local field potential recording showing spontaneous epileptiform events in rab11a crispants. Data were normalized to the mean values of the control group. Error bars indicate standard deviation. Data are shown as mean ± S.E.M. *P < 0.05, **P < 0.01.
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