FIGURE 1

Conservation and expression of zebrafish naxd and naxe. Alignment of human and zebrafish NAXD (A) and NAXE (B) protein sequences (human NAXD, NP_001229811.1; zebrafish Naxd, XP_005167521.1; human NAXE, NP_658985.2; zebrafish Naxe, NP_001002618.1). Strictly conserved residues and similar residues are highlighted in dark blue and light blue, respectively. Red asterisks indicate residues for which point mutations are known to cause NAD(P)HX repair deficiency in humans [3]. NAD(P)HX binding sites, ATP binding domains (NAXD), and potassium binding sites (NAXE) are highlighted by yellow, dashed orange, and pink boxes, respectively (based on UniProt feature annotation). mRNA expression levels of zebrafish naxd (blue) and naxe (pink) during the first 10 days post‐fertilization (relative to expression levels at 1dpf) (C) and in adult tissues (relative to expression levels in the skin) (D) measured by qPCR. Elfa (C) and rpl13 (D) were used as reference genes, respectively. Data are shown as means ± SDs from three to five (C) and six (D) biological replicates.