Fig. 3

Ultrastructure of outer retina in pikfyve crispants reveals vacuole accumulation. A-B) TEM images of 6 dpf uninjected control and pikfyve crispant zebrafish larvae, highlighting the buildup of vacuoles in the RPE of crispants, accompanied by expansion of the RPE (A), and the presence of large vacuoles in the crispant photoreceptor inner segments, just below the mitochondrial (m) cluster (B). Lower panels in A and B represent higher magnification views of areas shown in red boxes. C) Quantification of average number of vacuoles per 10 μm length of RPE in control uninjected and pikfyve crispant larvae. Each point represents an individual fish, averaged across multiple sections (n = 3 fish per group). D) Average area of RPE vacuoles for 3 fish per group. E) Quantification of area/length ratio of RPE provides measure of RPE expansion in pikfyve crispants compared to control fish. Each point represents the ratio for an individual fish, averaged across multiple sections (n = 3 fish per group). F) Inner segment (IS) vacuole area, pooled values measured in sections of retinas from three pikfyve crispant larvae.