Simultaneous deletion of and1 and and2 leads to absence of actinotrichia and fin-fold defects. (A) Transmitted light differential interference (TD) images of pectoral (pff) and median (mff) fin folds of wild type (n=5 larvae), and1−/−and2+/+ (n=3 larvae), and1−/−and2+/+ (n=3 larvae) and double mutants (n=5 larvae) at 5 dpf. Area outlined by solid white line is shown at a higher magnification below. The dashed black line outlines the fin fold (FF). Actinotrichia (black dotted arrows) are present in wild type but absent in double mutants. Wrinkling (white asterisk) is visible in the double mutant FF tissue. Scale bars: 100 µm. (B) And1- and Col2-immunostained wild-type sibling (n=6 larvae) and double mutant (n=6 larvae) posterior median FFs at 5 dpf. Dashed lines represent actinotrichia in the wild-type siblings. Actinotrichia are absent in the double mutant mff, shown by lack of And1 signal (a merged image of the fluorescent and transmitted light channels is shown), and by disorganized and aggregating Col2 (white asterisk). The FF margin is outlined in the Col2-immunostained double mutant. Scale bars: 100 µm. (C) Maximum intensity projection confocal images of ET37 wild-type (n=5 larvae) and double mutant (n=4 larvae) pff and mff at 5 dpf. Area outlined is shown at higher magnification on the right. Orange dashed arrows indicate the orientation of actinotrichia in the wild-type siblings. Scale bars: 200 µm; 50 µm in magnified image.
|
