ZFIN Image: Powell et al., 2012, Fig. 1

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Figure Caption

Fig. 1

Cloning of putative jama2 and jamc2 paralogues by 3? RACE or RT-PCR.

Agarose gel electrophoresis results of amplification of full-length jama2 by 3? RACE (A) or jamc2 by RT-PCR (B) from cDNA prepared from total RNA extracts of wild-type 24 h. p. f. zebrafish embryos. (A) Lanes: - 3? RACE negative control; 1?4 igsf11 positive control: 3? RACE ?1 5? primer, 2 nested 5? primer; nested PCR ?3 negative control, 4 nested 5? primer; 5?8 jama2:3? RACE ?5 5? primer, 6 nested 5? primer; nested PCR ?7 negative control, 8 nested 5? primer. (B) Lanes: - negative control; + ef1? positive control; 1 jamb positive control; 2 & 4 jamc2 primers designed to amplify predicted full-length ORF; 3 & 5 jamc2 primers designed to amplify IgSF domains. M, DNA size markers with size of selected bands indicated in kbp (left).

Figure Data

Expression / Labeling details

Acknowledgments

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