FIGURE

Fig. 1

ID

ZDB-FIG-120725-30

Publication

Powell et al., 2012 - Genomic organisation, embryonic expression and biochemical interactions of the zebrafish junctional adhesion molecule family of receptors

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

Cloning of putative jama2 and jamc2 paralogues by 3? RACE or RT-PCR.

Agarose gel electrophoresis results of amplification of full-length jama2 by 3? RACE (A) or jamc2 by RT-PCR (B) from cDNA prepared from total RNA extracts of wild-type 24 h. p. f. zebrafish embryos. (A) Lanes: - 3? RACE negative control; 1?4 igsf11 positive control: 3? RACE ?1 5? primer, 2 nested 5? primer; nested PCR ?3 negative control, 4 nested 5? primer; 5?8 jama2:3? RACE ?5 5? primer, 6 nested 5? primer; nested PCR ?7 negative control, 8 nested 5? primer. (B) Lanes: - negative control; + ef1? positive control; 1 jamb positive control; 2 & 4 jamc2 primers designed to amplify predicted full-length ORF; 3 & 5 jamc2 primers designed to amplify IgSF domains. M, DNA size markers with size of selected bands indicated in kbp (left).

Expression Data

Genes:	f11r.2 jam3a
Fish:	WT
Anatomical Term:	whole organism
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One