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Fig. 3
Runx/Cbfβ signaling is required to maintain runx expression in Rohon Beard neurons.
A-L, Colormetric in situ hybridization for runx1 (A-D) at 18s, runx3 (E-H) at 24hpf and 3dpf, and cbfb (I-L) at 24hpf and 3dpf in the runx1, runx3, and cbfb mutants focusing on the RBs. M-N, Quantification of marker gene expression as total number of RB neurons/embryo and as % WT at 18s or 24hpf. Arrow, RBs; Scale bar: 100 μm. Embryos per condition (n = 3–14). ***p<0.001. All error bars represent S.E.M.
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