Figure 5

Figure 5

GCGR regulates carbohydrate metabolism in zebrafish larvae. (A) Heatmaps of transcripts in carbohydrate metabolism enrichment. Colors represent high (red), low (blue), or average (white) expression values based on the Z-score normalized FPKM values for each gene. The Z-score indicators are shown under each map. The fold change (log2) are shown on the right. (B,C) 2-NBDG glucose uptake of wildtype (B) and gcgr^−/− mutant (C) larvae, the glucose uptake level is indicated by the fluorescence of lens (arrow) imaged by fluorescent microscopy. (D–E) Representative images of the principal islet of gcgr^−/−; Tg(gcga:GFP) (D) and 20 mM glucose treated-gcgr^−/−; Tg(gcga:GFP) (E). The images are confocal projections; scale bars indicate 10 μm. (F) Quantification of the α-cell number in different groups of zebrafish at 7 dpf, n ≥ 10. Ns: No significance, and ** p < 0.01 by t-test.