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Figure 4 Lens abnormalities in epha2b morphant zebrafish. To assess lens development, N-cadherin (red) and DAPI (blue) staining were performed on lens sections from wild-type (WT), epha2a, and epha2b morphant zebrafish at 2 dpf (a–c) and 3 dpf (d–f); this permitted visualisation of the lens fibre cells and cell nuclei, respectively. At 2 dpf, there were no apparent differences between the morphants and uninjected wild-type siblings. At 3 dpf, the epha2b morphant showed retention of fibre cell nuclei within the lens (f), which was not observed in age-matched wild-type (d) and epha2a morphant larvae. Scale bars = 25 µm.