FIGURE

Figure 4

ID

ZDB-FIG-210310-116

Publication

Harding et al., 2021 - EPHA2 Segregates with Microphthalmia and Congenital Cataracts in Two Unrelated Families

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Figure 4

Lens abnormalities in epha2b morphant zebrafish. To assess lens development, N-cadherin (red) and DAPI (blue) staining were performed on lens sections from wild-type (WT), epha2a, and epha2b morphant zebrafish at 2 dpf (a–c) and 3 dpf (d–f); this permitted visualisation of the lens fibre cells and cell nuclei, respectively. At 2 dpf, there were no apparent differences between the morphants and uninjected wild-type siblings. At 3 dpf, the epha2b morphant showed retention of fibre cell nuclei within the lens (f), which was not observed in age-matched wild-type (d) and epha2a morphant larvae. Scale bars = 25 µm.

Expression Data

Antibody:	Ab11-cdh2
Fish:	WT WT + MO1-epha2a WT + MO1-epha2b
Knockdown Reagents:	MO1-epha2a MO1-epha2b
Anatomical Term:	lens fiber cell adherens junction
Stage Range:	Long-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-epha2a WT + MO1-epha2b
Knockdown Reagents:	MO1-epha2a MO1-epha2b
Observed In:	lens fiber cell lens fiber cell nucleus lens fiber cell differentiation
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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