Loss function of claudin h caused the defects of otic vesicle and otoliths. (A,D) Imaging analysis of otic vesicle and otoliths in control and claudin h knocking down groups at 72 and 96 hpf. The yellow dotted line marked the boundary of the otic vesicle. Scale bar = 50 μm. (B,C) The statistical analysis of otic vesicle area in the control and claudin h morphants at 72 and 96 hpf. (E) Quantification of zebrafish embryos with abnormal otolith (defects in both number and shape of otoliths: claudin h morphants lost the utricle otolith or had a unnormal saccular otolith). Each bar represents the mean ± SE. Values with **** above the bars are significantly different (P < 0.0001).
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