Brain volume was not changed in zebrafish larvae by BPA exposure. A, 3D reconstruction of a larval brain. Sample confocal images of optical sections of a brain exposed to DMSO. Scale bar: 150 μm. B, Mean volumes of the forebrain (FB), midbrain (MB), and hindbrain (HB) in BPA-exposed (BPA group, n =15) fish compared with the vehicle-exposed fish (DMSO group, n =15). A two-way ANOVA revealed no significant interaction or group effect (interaction, F(2,84) = 0.03; p =0.75: group, F(1,84) = 0.10; p =0.97. C, Mean volume of the whole brain in BPA-exposed fish (BPA group, n =15) and vehicle-exposed fish (DMSO group, n =15). The difference between the two groups was not significant (t(28) = 0.23, p =0.82).
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