ZFIN Image: Schellens et al., 2023, Figure 2

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Figure Caption

Figure 2

Design and characterization of the ush2a^Δexon30-31 and ush2a^Δexon39-40 zebrafish line

(A) Schematic representation of the exon-excision approach. Sanger sequencing confirmed the presence of the anticipated excisions in injected embryos (1 day post fertilization [dpf]). Excision of the genomic region containing ush2a exons 30 and 31 resulted in the insertion of two nucleotides (TT) at the repair junction. (B) RT-PCR analysis revealed the absence of ush2a exons 30 and 31 in ush2a^Δexon30-31 larvae and the absence of exons 39 and 40 in ush2a^Δexon39-40 larvae (5 dpf). Sanger sequencing of the ush2a^Δexon30-31 and ush2a^Δexon39-40 amplicons confirmed the absence of the target exons from the transcript.

Acknowledgments

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