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Fig. 2 Nervous system developmental abnormalities caused by best1 knockdown. (A–L) Gross morphology of transgenic (HuC:EGFP) zebrafish embryos at 2-dpf. Compared with control morpholino (MO), knockdown of best1 gene causes head and brain patterning defects (E, I, blue arrowheads), microcephaly (E, I, yellow dotted line), small eyes (E, I, black arrowheads), brain-size reductions (F, J, H, and L), and abnormal neuronal outgrowth (F, J, asterisks). (M–O) Quantification of the head area (M), brain area (N) and average motor axon length (O) shows significantly decrease in best1 morphants. Columns, mean; standard error of the mean (n=10; analysis of variance), ***p < 0.0001. Scale bar, 100 μm. dpf, days post fertilization.