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Fig. 3 Earlier initiation of liver regeneration in male zebrafish is associated with earlier Yap1 activation in male livers following PH. (A) Expression of yap1 in zebrafish livers of both sexes at 6 h and 24 h post-PH as determined by RT-qPCR (n=3). (B,C) Expression of ctgfa, cyr61 and ankrd1b in zebrafish livers of both sexes at 6 h (B) and 24 h (C) post-PH as determined by real-time quantitative PCR (RT-qPCR) (n=4 for ankrd1b at 6 h, n=3 for the remaining groups). (D) Expression of ctgfa and cyr61 in zebrafish livers of both sexes at 48 h post-PH as determined by RT-qPCR (n=3). (E) IF staining of Yap1 in the zebrafish liver of both sexes at 6 h and 24 h post-PH. (F) Quantification of Yap1-positive liver cells based on E. Each symbol represents one zebrafish. Scale bars: 10 ?m. ns, not significant (P>0.05); *P?0.05, **P?0.01, ***P?0.001 (two-tailed unpaired Student's t-test).