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Fig. 6 Sex disparity in transcriptomic regulation during PH-induced zebrafish liver regeneration. (A) Workflow of differentially expressed gene analysis based on the RNA-sequencing results of PH-treated zebrafish livers of both sexes versus the corresponding sham-treated livers on Day 1 and Day 3. log2FC, log2 fold change. (B) Quantification of the significantly upregulated/downregulated genes identified in A. (C) The top 20 genes that were significantly upregulated only in male livers at 24 h post-PH. The green cross indicates significant downregulation. (D) Expression of s100a1 in zebrafish liver of both sexes following PH/sham surgeries as determined by RT-qPCR (n=3). (E) Expression of sox5, sox9a and sox9b in zebrafish liver of both sexes at 6 h post-PH as determined by RT-qPCR (n=3 for sox5, n=4 for the remaining groups). ns, not significant (P>0.05); *P?0.05, **P?0.01 (two-tailed unpaired Student's t-test).