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Fig. 7 Effects of s100a1 knockdown on liver expansion in zebrafish larvae during chemical injury-induced liver regeneration. (A) Experimental design for investigating the effects of s100a1 knockdown on liver regeneration of NTR+ larvae following metronidazole (MTZ)-induced hepatocyte ablation. NTR+ larvae injected with morpholino (MO) were exposed to 10 mM MTZ or DMSO for 24 h from 3.5 days post fertilization (dpf). (B) Fluorescence images of MO-injected NTR+ larvae following 24 h of DMSO or MTZ treatment. (C,D) Measurement and comparison of the area of CFP+ livers in MO-injected NTR+ larvae based on B. Each symbol represents one larva. (E) Expression of ctgfa and cyr61 in MO-injected NTR+ larvae 24 h after MTZ treatment as determined by RT-qPCR (n=3). Each sample used for quantitative PCR contained cDNA from 15 larvae. Scale bar: 200 ?m. *P?0.05, **P?0.01, ***P?0.001, ****P?0.0001 (two-tailed unpaired Student's t-test).