Figure 3

Figure 3

Still heart mutants have altered expression of key cardiac transcription factors and cardiac myosins. At 48 hpf, still heart mutants demonstrated a significant reduction in the relative fold change in expression of gata4, gata5, and anf compared to wild-type (A). Other key transcription factors, tbx5a, tbx5b, and tbx20, did not show a significant change in expression in still heart mutants, while the relative expression of tbx2b was elevated. Comparison of cardiac myosin heavy chain expression revealed no significant difference in vmhc expression between still heart and wild-type embryos at 24 hpf (B). Still heart mutants displayed a significantly higher relative fold change in amhc expression at 24 hpf. By 48 hpf, the relative fold change in expression of vmhc and amhc is significantly lower in still heart mutants when compared to wild-type (C). * = p ≤ 0.05, ** = p ≤ 0.01, *** = p ≤ 0.001.