Fig 2

Fig 2 taz mRNA and Taz protein are seen in the hindbrain during BVS development.

(A-A”). In situ hybridization of wild-type embryos stage 20 hpf show taz expression throughout the embryo, from the trunk and tail to broadly in the rostral region, shown both laterally (A) and dorsally (A’, A”). (A’) Dorsal view of the embryo showing taz expression in the somite region. (A”) Dorsal view of the embryo hindbrain showing taz expression is enriched at rhombomere boundaries. (B-B”). At 24 hpf, broad expression of taz in the anterior and in the trunk is still seen; taz mRNA enrichment at rhombomere boundaries also persists. (C) Taz protein is localized to rhombomere boundaries in wild-type 24 hpf embryos whereas (D) taz-/- mutants display only background signal.