Fig 4

Fig 4 Wnt activity affects taz expression at rhombomere boundaries.

(A-C’) In DMSO treated animals, at 24 hpf taz mRNA is seen broadly in the anterior of the embryo, with visible striations indicative of taz mRNA enrichment at rhombomere boundaries shown laterally (A) and dorsally with the hindbrain in focus (A’). In 5 µM XAV939 treated animals, at 24 hpf show a reduction of taz mRNA enrichment at the rhombomere boundaries (B-B’) or complete loss of this striated pattern (C-C’). (D) Injection of 200 pg Taz-Flag allowed visualization of the expected band for via western blot (lane 1; note the appearance of a non-specific band labeled *  present in all lanes, including the lane 5 negative control). Similarly, injection of 200pg Axin1-Myc resulted in a band at the expected MW being visualized via 9E10 western blot (lane 2). Co-injection of 200 pg Axin1-Myc and 200 pg Taz-Flag resulted in a greater amount of Flag-Taz detected (lane 3) which was also seen when 400 pg of Axin1-Myc and 400 pg Taz-Flag was injected (lane 4).