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Fig. 2 Clmp knockdown caused developmental defects during zebrafish embryogenesis. (A) The qRT-PCR analysis of relative expression levels of clmp in zebrafish embryos from 0.75 hpf to 120 hpf. (B) The qRT-PCR analysis of relative expression levels of clmp in control, control-MO, and clmp-MO zebrafish embryos at 24 hpf, 48 hpf, and 72 hpf (each group, n = 3). (C) The efficacy of clmp SBMO was confirmed by RT-PCR in embryos at 72 hpf. The larger products were observed when clmp SBMO was injected into the embryos (each group, n = 2). (D) Measurement of zebrafish body length. ib., intestinal bulb; mi, mid-intestine; pi, posterior intestine; *anal opening. (E) Statistical analysis of all groups' average body and gut lengths at 72 hpf. control(n = 16) versus control-MO (n = 16) versus clmp-MO (n = 22). clmp-MO, clmp morpholino group; control-MO, control morpholino group; SBMO, splice-blocking morpholino oligonucleotide; untreated group. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant.